System Configuration Microscope model: SP8 (Leica) Objectives: HC PL APO 20x/0.75 IMM CORR, CS2, No:11506343 HC FLUOTAR L 25x/0.95 W VISIR w. covergl, No:11506375 HC PL APO 40x/1.10 W, motCORR, CS2, No:11506360 HC PL APO 40x/1.30 Oil, CS2, No:11506358 HC PL APO 63x/1.20 W, CORR, CS2, No:11506346 HC PL APO 63x/1.40 Oil, CS2, No:11506350 HCX PL APO 100x/1.44 Oil, CORR CS, No:11506325 Source of light: EL6000 external light for fluorescence excitation “White” light pulsed laser with continues wavelength 470÷670 (nm) Lasers: 405 nm, 488 nm, 496 nm, 514 nm, 561 nm, 594 nm, 633 nm Filter cube: Leica N: 11513878, BZ:01, l3 Leica n 2.1 Leica N: 11513900, Analysator, B2 Leica N:11513873, BZ:04 Detectors: Hybrid Detector (3), PMT detector (2), Transmission PMT (1) Autofocus system: Adaptive focus, best focus Motorized: Stage, Z – galvo stage, objective turret, DIC prizms Live cell imaging: Stage top incubator, temperature controller with CO2 mixer Available Techniques Fluorescence imaging Time-Gating Phase contrast Differential interference contrast Z stack Image stitching
