Laser Scanning Confocal

System Configuration

Microscope model:

SP8 (Leica)


HC PL APO 20x/0.75 IMM CORR, CS2, No:11506343
HC FLUOTAR L 25x/0.95 W VISIR w. covergl, No:11506375
HC PL APO 40x/1.10 W, motCORR, CS2, No:11506360
HC PL APO 40x/1.30 Oil, CS2, No:11506358
HC PL APO 63x/1.20 W, CORR, CS2, No:11506346
HC PL APO 63x/1.40 Oil, CS2, No:11506350
HCX PL APO 100x/1.44 Oil, CORR CS, No:11506325

Source of light:

EL6000 external light for fluorescence excitation
“White” light pulsed laser with continues wavelength 470÷670 (nm)
Lasers: 405 nm, 488 nm, 496 nm, 514 nm, 561 nm, 594 nm, 633 nm

Filter cube:

Leica N: 11513878, BZ:01, l3
Leica  n 2.1
Leica N: 11513900, Analysator,  B2
Leica N:11513873, BZ:04


Hybrid Detector (3), PMT detector (2), Transmission PMT (1)

Autofocus system:

Adaptive focus, best focus


Stage, Z – galvo stage, objective turret, DIC prizms

Live cell imaging:

Stage top incubator, temperature controller with CO2 mixer

Available Techniques

Fluorescence imaging


Phase contrast

Differential interference contrast

Z stack

Image stitching