Widefield Fluorescence Microscope
Widefield Fluorescence Microscope
System Configuration
Microscope model
DMi8, Inverted
Objective Lenses
DRY – air refractive index 1.0003
WATER- refractive index 1.330
OIL- refractive index 1.5180 (23 C)
IMM – refractive index 1.330 ≤ n ≤ 1.5180
Resolution (XY, Z)- for wavelength 550nm
Working Distance – distance between Objective Lens and Cover Glass
Phase Ring – Condenser Annulus for Phase Contrast imaging
IC Prism – the Wollaston prism at the objective lens for DIC imaging
Cond. Prism DIC - the Wollaston prism at the condenser for DIC imaging
|
Type |
HC PL FLUOTAR |
Working Distance (um) |
11000 |
|
Magnification |
10 |
Phase Ring |
PH1 |
|
Numerical Aperture |
0.30 |
IC Prisms |
D1, D, D1-P |
|
Immersion |
DRY |
Technique |
PH1 |
|
Resolution XY (um) |
1.118 |
Cond. Prism DIC |
K3, K11 |
|
Resolution Z (um) |
6.111 |
Number |
11506507 |
|
Type |
HC PL FLUOTAR |
Working Distance (um) |
33000 |
|
Magnification |
40 |
Phase Ring |
PH2 |
|
Numerical Aperture |
0.60 |
IC Prisms |
C1,C,C2,C1-P |
|
Immersion |
DRY |
Technique |
PH2 |
|
Resolution XY (um) |
0.559 |
Cond. Prism DIC |
K6, K8, K16 |
|
Resolution Z (um) |
1.528 |
Number |
11506203 |
|
Type |
HC Pl FLUOTAR |
Working Distance (um) |
2400 |
|
Magnification |
25 |
Phase Ring |
- |
|
Numerical Aperture |
0.95 |
IC Prisms |
D1 |
|
Immersion |
WATER |
Technique |
- |
|
Resolution XY (um) |
0.353 |
Cond. Prism DIC |
K4 |
|
Resolution Z (um) |
0.811 |
Number |
11506375 |
|
Type |
HC PL APO CS2 |
Working Distance (um) |
90 |
|
Magnification |
63 |
Phase Ring |
PH3 |
|
Numerical Aperture |
1.40 |
IC Prisms |
E |
|
Immersion |
OIL |
Technique |
CS2 |
|
Resolution XY (um) |
0.240 |
Cond. Prism DIC |
K10 |
|
Resolution Z (um) |
0.426 |
Number |
11506350 |
|
Type |
HC PL APO |
Working Distance (um) |
100 |
|
Magnification |
100 |
Phase Ring |
- |
|
Numerical Aperture |
1.40 |
IC Prisms |
D |
|
Immersion |
OIL |
Technique |
- |
|
Resolution XY (um) |
0.240 |
Cond. Prism DIC |
K10 |
|
Resolution Z (um) |
0.426 |
Number |
11506381 |
Multi-color source of light
Lumencor SpectraX, instslled filters (nm): 395/25, 440/20-25, 470/24-25, 510/25-25, 575/25-25, 640/30-25.
Available extra filter 550/15 (nm).
Transmitted light
Intensity, polarization, and aperture controlled white light
Filter cubes
|
Turret Position |
Cube Name |
Excitation (nm) |
Dichroic (nm) |
Emission (nm) |
Leica Number |
|
1 |
DAPI395 |
395/25 |
LP 425 |
BP 460/50 |
11533333 |
|
2 |
GFP |
470/40 |
495 |
500-550 |
11525314 |
|
3 |
CFP-YFP-mCherry |
436/20, 503/21, 575/25 |
455, 520, 595 |
470/25, 535/30, 635/70 |
Chroma custom |
|
4 |
YFP |
510/25 |
> 530 |
550/30 |
Chroma custom |
|
5 |
ANALIT-TL |
- |
- |
- |
11525300 |
|
6 |
FM4-64 |
575/25 |
>610 |
>610 |
Chroma custom |
|
not installed |
Quad Led |
395/25 470/24-25 575/25-25 640/30-25 |
415, 490 570; 660 |
412-448 495-535 575-615 670-770 |
Chroma |
Chroma CFP-YFP-mCherry (excitation 436/20, 503/21, 575/25)
Camera
Andor EMCCD iXon Ultra 888 EX, number of pixels 1024x1024, 16 Bit digital signal
Control Software
Leica Application Suite X (LAS X)
Stage Top Incubator
Tokai Hit, model: INUBG2ATFP-WSKM
Precision control of temperature (37C), gas mix CO2 (5%), and humidity (90%)
Autofocus system
Adaptive focus, Fast focus
Motorized
XY Stage, Z focus, objective turret, DIC prisms
Available Imaging Techniques
Fluorescence imaging
White light imaging
Phase contrast
Differential interference contrast
Focus/Z - stacking
3D image deconvolution
Image stitching
Stage multi-positioning
Time - lapse